Example sentences of "v c [noun] [letter] " in BNC.
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| 1 | Voltages applied to the membrane patches ( V c o m ) were referenced to the interior of the patch pipette . |
| 2 | With high K + solution in the bath , cell membrane voltage was assumed to be zero , and V c o m was therefore equivalent to the potential across the membrane patch in both the cell attached and inside out configurations . |
| 3 | Current-voltage ( I-V c o m ) relations were constructed by plotting the single channel current ( equal to the difference between the open and closed channel currents ) at each value of V c o m . |
| 4 | Single channel slope conductance was calculated at two values of V c o m : ( a ) at -40 mV , the cell membrane voltage measured in rabbit gastric glands with conventional microelectrodes and in rabbit parietal cells during whole cell current clamp studies , and ( b ) at infinite voltage ( corresponding to the linear portion of the I-V c o m curve ) where channel conductance was maximal . |
| 5 | The sensitivity of K + channels to Ca 2 + ions was determined in excised inside out patches by recording ( V c o m =0 mV ) first in the presence of the Ca 2 + containing high K + bath solution , and then in the presence of a Ca 2 + free high K + solution containing the Ca 2 + chelator ethyleneglycol-bis ( Β -aminoethyl ether ) N , N' tetra-acetic acid ( EGTA , 5 mM ) . |
| 6 | With NaCl solution in the bath and high K + solution in the pipette , recordings were obtained from cell attached patches ( V c o m = 0 mV ) before and after the addition of 0.1 mM dibutyryl cAMP ( a permanent analogue of cAMP ) or 6.7 µM A23187 ( a Ca 2 + ionophore ) . |
| 7 | Under basal conditions , spontaneous channel activity was seen at V c o m =0 mV in 6 of 35 ( 17% ) cell attached patches and in 14 of 85 ( 16% ) excised inside out patches . |
| 8 | Under these conditions ( high K + bath solution , NaCl pipette solution ) , progressive depolarisation of the membrane patch ( increasingly positive V c o m ) increased the amplitude of the outward K + currents , and Fig 1B shows the I-V c o m curve for this channel . |
| 9 | Mean single channel conductances at V c o m =-40 mV ( g - 4 ) and V c o m =infinity were 56 ( 6 ) pS and 155 ( 23 ) pS respectively , the mean value of E r e v was -84.2 ( 0.2 ) mV , and inward currents were not seen when V c o m was varied between -80 mV and -120 mV . |
| 10 | Mean single channel conductances at V c o m =-40 mV ( g - 4 ) and V c o m =infinity were 56 ( 6 ) pS and 155 ( 23 ) pS respectively , the mean value of E r e v was -84.2 ( 0.2 ) mV , and inward currents were not seen when V c o m was varied between -80 mV and -120 mV . |
| 11 | Mean single channel conductances at V c o m =-40 mV ( g - 4 ) and V c o m =infinity were 56 ( 6 ) pS and 155 ( 23 ) pS respectively , the mean value of E r e v was -84.2 ( 0.2 ) mV , and inward currents were not seen when V c o m was varied between -80 mV and -120 mV . |
| 12 | Figure 3 shows the relation between single channel open probability ( P o ) and pipette potential ( V c o m ) in excised inside out patches ( high K + bath solution , NaCl pipette solution ) . |
| 13 | In contrast , with inside out patches ( for example , Fig 2A ) channels generally spent longer in the open state at equivalent values of V c o m , while closed times were similar to those seen in cell attached patches ( see below ) . |
| 14 | Fibure 4A shows typical histograms from one of four cell attached patches , which showed ( at V c o m =0 mV ) one open time constant ( mean value 6.7 ( 1.0 ) ms ) and two closed times constants ( mean values 0.7 ( 0.2 ) ms and 4.2 ( 1.1 ) ms ) , with mean open and closed times of 6.8 ( 0.7 ) ms and 3.0 ( 0.5 ) ms respectively . |
| 15 | Figure 4B shows histograms from one of five inside out patches , which again showed ( at V c o m =0 mV ) one open time constant ( mean value 31.1 ( 10.0 ) ms ) and two closed time constants ( mean values 0.4 ( 0.1 ) ms and 1.8 ( 0.3 ) ms ) , with mean open and closed times of 30.4 ( 8.1 ) ms and 1.5 ( 0.2 ) ms respectively . |
| 16 | Consequently , Ca 2 + sensitivity of the K + channel was examined in only two inside out patches at V c o m =0 mV . |
| 17 | Thus , although it was not possible to obtain a detailed analysis of the relation between P o and V c o m at different Ca 2 + concentrations , the available data show that under these experimental conditions , K + channels were sensitive to changes in the concentration of Ca 2 + bathing the cytosolic surface of the membrane . |
| 18 | The effect Ba 2 + ions ( final bath concentration 5 mM ) on K + channel activity was examined in inside out patches ( V c o m =0 mV ) . |
| 19 | With NaCl solution in the bath and high K + solution in the pipette ( V c o m =0 mV ) , the addition of 0.1 mM dibutyryl cAMP failed to elicit K + channel activity in 17 cell attached patches while recording for periods of up to 15 minutes , suggesting that K + channels in HGT-1 cells are not regulated by intracellular camp . |
| 20 | In contrast , the assition of 6.7 µM A23187 stimulated K + channel activity ( generally within four minutes , see Fig 7 ) in five of 24 ( 21% ) quiescent cell attached patches , P o increasing to 0.218 ( 0.097 ) at V c o m =0 mV . |
| 21 | It seems extremely unlikely that the inward currents reflect Cl - movement from the cell to the pipette by Ca 2 + -activated Cl - channels , as the mean single channel current at V c o m =0 mV ( 5.5 ( 1.3 ) pA , n=5 ) was considerably greater than that ( approximately 1 pA at V c o m =0 mV ) flowing through cAMP activated Cl - channels in cell attached patches of HGT-1 cells with NaCl solution in both the bath and the pipette . |
| 22 | It seems extremely unlikely that the inward currents reflect Cl - movement from the cell to the pipette by Ca 2 + -activated Cl - channels , as the mean single channel current at V c o m =0 mV ( 5.5 ( 1.3 ) pA , n=5 ) was considerably greater than that ( approximately 1 pA at V c o m =0 mV ) flowing through cAMP activated Cl - channels in cell attached patches of HGT-1 cells with NaCl solution in both the bath and the pipette . |