Example sentences of "and 10 [unc] " in BNC.

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1 B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c .
2 B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c .
3 B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c .
4 B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c .
5 Maintenance of procyclic trypanosomes : T.brucei strain EATRO 1125 were grown at 25-27°C in SDM-79 ( 18 ) , or Cunningham 's , medium ( 19 ) , supplemented with 15% inactivated foetal calf serum and 10 µg ml -1 gentamycin .
6 Addition of 200 nM oxyhaemoglobin and 10 µM methylene blue to the organ bath , one and 10 minutes before cells respectively , reduced muscle relaxation .
7 Postprandial concentrations reach 5 mmol/l in the jejunum and 10 mmol/l in the ileum .
8 After incubation for 30 minutes at 42°C , the reaction was chilled to 4°C and 10 µl of 0.5 KGB buffer plus 10 units of E.coli DNA polymerase 1 and 0.4 units of RNaseH was added .
9 In all lanes 2 µl of a cDNA synthesis was amplified and 10 µl of the PCR product was electrophoresed on a 1% agarose gel .
10 Immunoprecipitation with mABs was carried out as described by Sonnenberg et al with the modification : cell extracts were first incubated overnight at 4°C with 10 µl of the specific antibody and 10 µl of a species specific polyclonal rabbit lgG ( Dako , Hamburg , Germany ) antibody and then for an incubation period of one hour with 9 mg protein A sepharose at 4°C .
11 Muscle relaxation by cells was reduced by 200 nM oxyhaemoglobin and 10 µ M methylene blue but was increased by 60 n/ml superoxide dismutase .
12 On the day of transfection , the medium was replaced with 3 ml αMEM plus 1% FBS containing 10 µg plasmid DNA and 10 µg/ml of poly-L-ornithine ( Sigma , P3655 ) , dispensed from a 10 mg/ml sterile water stock solution , and incubated at 37°C with gentle mixing every 1.5 hours .
13 Hyperamylasaemia and hyperlipasaemia were defined as increases above the upper limits of normal , which were 52 IU/l and 200 IU/l respectively , before January 11 , 1989 , and 10 IU/l and 208 IU/l , respectively , after January 11 , 1989 .
14 Ninety per cent are very poor and 10 per cent are very rich . ’
15 Marcos , it is said , tried to set up a foundation last year to launder - sorry , deposit — the funds to benefit Filipinos , minus a 10 per cent cut for his greedy relatives and 10 per cent for the Vatican .
16 The two cities indirectly hold more than 17 per cent and 10 per cent of MBB which could be enough to stop the merger , observers said .
17 Twenty per cent of smokers in the MORI surveys deny passive smoking is harmful , and 10 per cent believe smoking does not cause fatal diseases .
18 Volvo now owns 20 per cent of Renault 's car division , with the French company owning 25 per cent of Volvo Cars and 10 per cent of AB Volvo .
19 It is available on home loans of between £40,000 and £150,000 , where payments are deferred on 20 per cent of the loan in the first year and 10 per cent in the second .
20 In the constituency section figures are less certain , but Mr Davies 's supporters calculate around 23 per cent for him , 27 per cent forMr Field and 10 per cent for Ms Wilson .
21 Experiments in pickling different alloys of copper and zinc have shown that only alloys with between 2 and 10 per cent of copper in the zinc will take on the black patina , and the silver and brass inlays are unaffected by the pickling solution .
22 Thus , if one had a bronze with , say , 2 per cent of tin , where the tin content of the comparable genuine bronzes lay between 5 and 10 per cent , this would be unusual and would count against the piece but it certainly would not condemn it .
23 Britain , with a defence budget of £1,500 million a year , was actually spending an even higher proportion of its wealth on arms — between 8 per cent and 10 per cent of the Gross National Product , a third of its tax revenue .
24 In this country between 5 and 10 per cent of cases of gonorrhoea in women go on to develop a degree of tubal involvement , and only a few of these will lead to peritonitis .
25 There will be spots or sores on the mucous membranes in one third of cases , and 10 per cent will have signs of involvement of other parts of the body including the eye , nervous system , bones , or internal organs .
26 On the other hand , if there are no others in the field , an offer of , say , 2½ per cent less than the asking price could be made ; and if the house is somewhat overpriced , try an offer between 5 and 10 per cent less than the asking price and see what the reaction is .
27 By 1885 Nottingham had over 2,000 machines at work in steam-powered factories , and 10 per cent of the whole county 's population was employed in the lace industry .
28 He eventually found that the ideal mix was 55 per cent Polled Lincoln Red , 35 per cent Polled Beef Shorthorn ( he had also succeeded in polling the latter ) and 10 per cent Aberdeen Angus , which improved the qualities he desired without reducing size and liveweight gains .
29 Savannas also occupy 60 per cent of Australia , 45 per cent of South America and 10 per cent of India and Southeast Asia ( MM Cole 1986 ) .
30 It contributed no more than 20 per cent of the lead in the blood of adults and 10 per cent in children .
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