Example sentences of "10 [unc] " in BNC.
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| 1 | Yet , choline output remained below 10 µg/h showing that lipidic material purified from gastric juice was not of biliopancreatic origin . |
| 2 | SCFAs were extracted into ether from acidified aliquots of faeces after the addition of isovaleric acid ( 10 µmol per g faeces ) as an internal standard . |
| 3 | In a genomic Southern blot it is impractical to load more than 10 µg ( 10 -6 g ) of restriction enzyme digested DNA per lane of an agarose gel . |
| 4 | These cells were transfected by the CaPO 4 -DNA precipitate method [ 16 ] with 10 µg test gene plasmid , 1 µg reference gene plasmid and the indicated amount of either trans-activator plasmid or herring sperm DNA overnight . |
| 5 | B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c . |
| 6 | B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c . |
| 7 | B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c . |
| 8 | B Autoradiograph of an RNAse protection assay with RNA of transfected HeLa cells and C. autoradiograph of an EMSA performed with nuclear extracts of these transfected cells to monitor the amount of expressed proteins. lane 1 : cells transfected with DNAs d , f ; lane 2 : cells transfected with DNAs d , f and 5 µg DNA a ; lane 3 : cells transfected with DNAs d , f and 10 µg DNA a ; lane 4 : cells transfected with DNAs d , f and 5 µg DNA b ; lane 5 : cells transfected with DNAs d , f and 10 µg DNA b ; lane 6 : cells transfected with DNAs d , f and 5 µg DNA c ; lane 7 : cells transfected with DNAs d , f and 10 µg DNA c ; lane 8 : cells transfected with DNAs d , f and 5 µg DNA b and 5 µg DNA c ; lane 9 : cells transfected with DNAs d , f and 5 µg DNA b and 10 µg DNA c ; lane 10 : cells transfected with DNAs e , f ; lane 11 : cells transfected with DNAs e , f and 5µg a , lane 12 : cells transfected with DNAs e , f and 5µg b , lane 13 : cells transfected with DNAs e , f and 5µg c . |
| 9 | Duplex DNA , prepared and [ 32 P ] -5'-endlabelled as described above , was mixed with 10 µg Dcm or Dcm/Cys177Ser protein in 20 mM Tris-HCl , 1 mM EDTA , 10 mM β-mercaptoethanol , 50 mM NaCl , pH 7.5 in a total volume of 20 µl . |
| 10 | [ 32 P ] -5'-endlabelled or non-radioactive duplex DNA , prepared as described above , was mixed with 10 µg Dcm or Dcm/Cys177Ser protein in DCM reaction buffer . |
| 11 | Genomic DNA from D.melanogaster adult flies ( 10 µg ) was digested with Hin dIII ( 1 ) , Pst I ( 2 ) , Bam HI ( 3 ) and Eco RI ( 4 ) and hybridized with S2-ribosomal protein specific probe . |
| 12 | Filtered ( through 0.22 µm syringe filters ) supernatants are subjected to two sequential organic extractions and DNAs are recovered by precipitation with ethanol in the presence of 10 µg carrier glycogen . |
| 13 | On the day of transfection , the medium was replaced with 3 ml αMEM plus 1% FBS containing 10 µg plasmid DNA and 10 µg/ml of poly-L-ornithine ( Sigma , P3655 ) , dispensed from a 10 mg/ml sterile water stock solution , and incubated at 37°C with gentle mixing every 1.5 hours . |
| 14 | Standard transfections were carried out using 10 µg of DNA per 2 10 6 cells on a 90 mm plate . |
| 15 | In experiments where the effect of Oct-1 on gene expression was being assessed , 10 µg of the reporter plasmid was co-transfected with the indicated amounts of the Oct-1 expression vector with the amount of transfected DNA in each sample being equalized with the parental plasmid . |
| 16 | CAT assay of extracts prepared by the transfection of BHK-21 cells with pBL 2 CAT containing the HPV 16 octamer/NFI motif in the presence of 10 µg of plasmid vector alone ( track 1 ) , 10 µg of the RSV-NF4 expression vector ( track 2 ) , 10 µg of the RSV-NF21 expression vector ( track 3 ) , 5 µg of the RSV-NF4 expression vector with 5 µg of the Oct-1 expression vector ( track 4 ) and 5 µg of the RSV-NF21 expression vector with 5 µg of the Oct-1 expression vector ( track 5 ) . |
| 17 | CAT assay of extracts prepared by the transfection of BHK-21 cells with pBL 2 CAT containing the HPV 16 octamer/NFI motif in the presence of 10 µg of plasmid vector alone ( track 1 ) , 10 µg of the RSV-NF4 expression vector ( track 2 ) , 10 µg of the RSV-NF21 expression vector ( track 3 ) , 5 µg of the RSV-NF4 expression vector with 5 µg of the Oct-1 expression vector ( track 4 ) and 5 µg of the RSV-NF21 expression vector with 5 µg of the Oct-1 expression vector ( track 5 ) . |
| 18 | CAT assay of extracts prepared by the transfection of BHK-21 cells with pBL 2 CAT containing the HPV 16 octamer/NFI motif in the presence of 10 µg of plasmid vector alone ( track 1 ) , 10 µg of the RSV-NF4 expression vector ( track 2 ) , 10 µg of the RSV-NF21 expression vector ( track 3 ) , 5 µg of the RSV-NF4 expression vector with 5 µg of the Oct-1 expression vector ( track 4 ) and 5 µg of the RSV-NF21 expression vector with 5 µg of the Oct-1 expression vector ( track 5 ) . |
| 19 | Lyophilised preparations of myeloperoxidase ( Calbiochem , La Jolla , CA , USA ) , human milk lactoferrin ( Sigma Chemical Co , St Louis , MO , USA ) , and bovine milk lactoferrin ( Sigma ) were dissolved in carbonate-bicarbonate buffer ( 10 µg antigen/ml ) and used for ELISA . |
| 20 | Adenylate cyclase in thepellet was determined by adding 15 µl of homogenate ( 10 µg protein ) to 235 µl of incubation buffer to give the following final concentrations in the assay mixture ( pH 7.5 ) : 25 mmol/l Tris HCl , 1 mmol/l EGTA , 0.06 mmol/l EDTA 0.06 mmol/l dithiothreitol , 1.8 mmol/l NaCl , 5 mmol/l MgCl 2 , 1 mmol/l ATP , 20 mmol/l creatine phosphate , 0.1 mg/ml creatine kinase , 0.2 mmol/l IBMX , 1 mg/ml bovine serum albumin , 200 µg/ml bacitracin , and 200 µmol/l GTP . |
| 21 | Further studies showed that this effect on anomal weight and pellet consumption was not seen with the addition of porcine colipase alone and that there was a significant reduction in total weight of food consumed at six hours when 10 µg synthetic VPDPR was given intraperitoneally . |
| 22 | In vitro kinase assays were performed in a 30 µl reaction which contained the following ; 10 µg HeLa nuclear extract ( 28 ) or 200 ng of purified DNA-PK ( a gift from C.Anderson ) , 250 ng substrate protein , 25 mM Hepes pH 7.5 , 12.5 mM MgCl 2 , 20% glycerol , 0.1% NP-40 , 100 mM KCl , 50 µM ATP ( containing 10 µCi [ γ 32 P ] ATP ; 6000 Ci/mmol ) and 1 mM DTT. 200 ng of linearised DNA template was added to the appropriate reaction as indicated in the text . |
| 23 | Maintenance of procyclic trypanosomes : T.brucei strain EATRO 1125 were grown at 25-27°C in SDM-79 ( 18 ) , or Cunningham 's , medium ( 19 ) , supplemented with 15% inactivated foetal calf serum and 10 µg ml -1 gentamycin . |
| 24 | Genomic DNA ( 10 µg ) from the ura5-6 strain was digested with different restriction enzymes , electrophoresed on a 0.8% agarose gel , transferred to nylon membrane , and probed with 32 P-labeled ( T 2 AG 3 ) 4 . |
| 25 | Genomic DNA from the ura5-6 strain was treated with Bal 31 nuclease for 0 ( lane 1 ) , 0.5 ( lane 2 ) , 2 ( lane 3 ) , 7.5 ( lane 4 ) , 15 ( lane 5 ) , or 60 min ( lane 6 ) and subsequently digested with Sal I. ( A ) DNA samples ( 10 µg ) were electrophoresed on a 0.8% agarose gel , transferred to nylon membrane , and hybridized with 32 P-labeled ( T 2 AG 3 ) 4 . |
| 26 | On the day of transfection , the medium was replaced with 3 ml αMEM plus 1% FBS containing 10 µg plasmid DNA and 10 µg/ml of poly-L-ornithine ( Sigma , P3655 ) , dispensed from a 10 mg/ml sterile water stock solution , and incubated at 37°C with gentle mixing every 1.5 hours . |
| 27 | The final concentration of heparin was 10 µg/ml , and that of the NTPs , 200 µM . |
| 28 | After 10 min , heparin was added up to 10 µg/ml and incubation continued for either 15 seconds or 10 min ( indicated in the figure as +h and +H , respectively ) . |
| 29 | In addition to its ADP and arcachidonic acid independent aggregating activity on rabbit platelets , the lipidic material was further characterised as platelet activating factor on the basis of the following criteria : ( a ) its aggregating activity in the presence of 0.1 mM BN 52021 , a specific platelet activating factor receptor antagonist ; ( b ) its aggregating activity after incubation of the samples with 10 µg/ml phospholipase A 2 from hog pancreas and with 100 µg/ml lipase A 1 from R arrhizus ; ( c ) its retention time during high performance liquid chromatography analysis using phosphatidylcholine , lysophosphatidylcholine , and synthetic C-18 platelet activating factor as standards . |
| 30 | Monocytes from healthy volunteers were isolated by adherence to bacteriological petri dishes , after a Ficoll Paque ( Pharamacia ) sedimentation step , cultured in PRMI 1640 medium at 10 6 /ml concentration for at least four hours and then stimulated with LPS ( Sigma ) 10 µg/ml . |