Example sentences of "[verb] as [verb] previously " in BNC.
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| 1 | Cytoplasmic cell extract ( S100 ) was prepared as described previously ( 7 ) . |
| 2 | Pancreatic secretion was examined in rats with chronic pancreatic fistula prepared as described previously . |
| 3 | Isolated pancreatic acini were prepared as described previously by collagenase digestion of the pancreas obtained from saline treated rats and those receiving infusion of caerulein alone , platelet activating factor alone , TCV-309+ caerulein or TCV+platelet activating factor . |
| 4 | Oxyhaemoglobin was prepared as described previously , by reduction of bovine haemoglobin ( Sigma ) with sodium hydrosulphite ( 10-fold molar excess ) followed by gel filtration with a prepacked disposable column ( PD-10 , Pharmacia ) , previously equilibrated with buffered Krebs solution . |
| 5 | The in vitro transcription reactions were performed as described previously for classical pol III genes ( 19 ) and for the U6-RNA gene ( 5 ) . |
| 6 | SDS-PAGE of Ga lamblia antigens on 10% gels followed by electrophoretic transfer to nitrocellulose and immunoblotting were performed as described previously . |
| 7 | ( 2 ) The handling of native and model biles , cholesterol labelling and chromatographic separation to vesicular and non-vesicular phases were performed as described previously . |
| 8 | Preparation of plasma for determination of isotopic enrichment of free leucine was performed as described previously . |
| 9 | Gel retardation assays were performed as described previously ( 24 ) . |
| 10 | Gel retardation analysis was performed as described previously ( 11-13 ) . |
| 11 | Rabbit platelets were washed as described previously : aspirinated platelets ( 1.6×10 8 ) in 300 µl Tyrode 's solution , containing 2.5% gelatin and the ADP scavenger mixture creatine phosphate ( 1 mM ) and creatine phospholinase ( 10 IU/ml ) were stirred in an aggregometer ( Icare , Marseille , France ) . |
| 12 | HGT-1 cells were cultured as described previously . |
| 13 | Spodoptera frugiperda cells ( IPLB-SF21 ) , AcNPV and recombinant viruses were propagated as described previously ( 11 , 12 ) . |
| 14 | The systolic , diastolic , and mean arterial pressures were recorded as described previously with an automatic device ( Dinamap , Critikon , Florida ; cuff size 23–13 cm/13 cm ) every three minutes for 30 minutes between 0900 and 1100 while the patient was supine , and the mean of these 10 values was recorded . |
| 15 | 7α-Hydroxy-4-cholesten-3-one was analysed as described previously . |
| 16 | The pancreatic secretion was collected from the pancreatic fistula in small preweighed vials in 30 minute aliquots to measure the volume flow and protein outputs as described previously . |
| 17 | UNIT The descriptive name of the media unit onto which the media item should be placed as defined previously by the Offline Manager using option 9.4.0 . |