Example sentences of "[verb] [conj] previously [vb pp] " in BNC.
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| 1 | RNA polymerases : After isolation of each RNA polymerase subunits ββ ’ and α as well as truncated a-subunits α-235 and α-256 , three different core enzymes containing the wild-type α-subunit or the truncated α-235 or α-256 subunits were reconstituted and purified as previously described [ 13 ] . |
| 2 | Protoplasts from the ura5-6 strain were prepared and transformed as previously described ( 28 , 29 ) . |
| 3 | RT-based PCR was done as previously described . |
| 4 | HPLC was used to determine 5-ASA and Ac-5-ASA in serum and in biopsy specimen homogenates as previously described . |
| 5 | Single-cell suspensions of BALB/c EC were prepared as previously described , except that trypsin digestion was done in Hank 's balanced salt solution ( HBSS ) containing 1mM calcium ( Biofluids ) , 10mM HEPES ( GIBCO BRL ) . |
| 6 | The animals were sacrificed 60min later and optic nerve cell suspensions were prepared as previously described . |
| 7 | Assay for platelet activating factor — Washed rabbit platelets were prepared as previously described . |
| 8 | Nuclear extracts were prepared as previously described ( 29 ) . |
| 9 | The pUC19 polylinker containing a dimeric insert of T 9 GCA 9 , cloned into the Sma I site , was prepared as previously described [ 25 ] and labelled at the 3'-end of the Hin dIII site . |
| 10 | Over all the years , including these nineteen , some bonds were given to the club but the majority were bequeathed to individuals , either to be kept as an investment out of family sentiment , or possibly sold quietly to Club members , selected as previously described . |
| 11 | It is thought that previously matured vitrinite would begin to undergo maturation again once it reached the maximum temperature to which it had been originally exposed . |
| 12 | Lyso platelet activating factor was chemically acetylated into platelet activating factor , extracted in ethanol and measured as previously described . |
| 13 | The synthesis and purification of all oligonucleotides and their characterisation by enzymatic digestion was performed as previously reported ( 3 ) . |
| 14 | S1 nuclease mapping of start sites for transcription and purification and sequencing of 32 P-labelled fragments were performed as previously described ( 14 ) . |
| 15 | ELISAs were performed as previously described . |
| 16 | These were performed as previously described . |
| 17 | Detection of CREB by Western blotting and immunoprecipitation of 32 P-labelled CREB and ATF1 was performed as previously described ( 29 ) . |
| 18 | DNase I footprinting reactions were performed as previously described [ 10 , 27-29 ] . |
| 19 | The animals were anaesthetised three hours later and gastric blood flow and area of gastric lesions were measured as previously described . |
| 20 | The serum IGF-I concentration was measured as previously described by radioimmunoassay ( RIA ) after acid ethanol extraction using a rabbit antiserum developed by L U Underwood and J J Van Wyk and distributed by the Hormone Distribution Program of NIDDK through the National Institutes of Health , Bethesda , MD , USA . |
| 21 | Mitochondria from adults flies ( 1 to 8 days ) were isolated as previously described ( 17 ) . |
| 22 | In-vitro production of anti-toxoplasma and anti-HIV-1 by PBL was detected as previously described . |
| 23 | As with a number of other computer companies , Pick has discovered that there are brains to be hired that previously worked in academia , the military , government and even the KGB . |
| 24 | These results suggest either that 5HT receptors are more widely distributed than previously recognised or that sumatriptan is less specific in its agonist activity . |
| 25 | Transmission will stop only after the XOFF character has been recognised and , since the Z88 has an input buffer , this can only happen after previously received characters have been processed . |
| 26 | The need to establish the legitimacy of the political system after the years of repression and apathy meant that previously excluded groups such as the organized working class had to be brought into the political process . |
| 27 | It is concluded that previously reported values for trace metals in gastric juice have been incorrect and that the very low amounts secreted in the gastric juice will not interfere with the absorption of trace metals from the diet . |
| 28 | Proceed as previously informed . |
| 29 | Two 7.5 ml urine samples from one male subject at 75 and 90 minutes after the onset of the meal , collected and stored as previously described , were thawed at room temperature , pooled , lyophilised , and then reconstituted by mixing with 6 ml distilled water . |
| 30 | Proteins were electrophoresed on denaturating SDS/polyacrylamide gels containing 17.5% acrylamide/bisacrylamide and visualized by Coomassie brilliant blue R250 staining as previously described ( 7 ) . |