Example sentences of "were [vb pp] as described [prep] " in BNC.

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1 R H /R W ratios were calculated as described in Material and methods .
2 Bacterially expressed subunits of TFIIF , r74 and r30 , were purified as described in Materials and Methods , and analyzed on 10% SDS-polyacrylamide gel electrophoresis .
3 Reaggregate cultures were prepared as described for Fig. 1 .
4 In a , optic nerve sections were prepared as described in Fig. 1 .
5 The employed protein fractions were prepared as described in Materials and Methods .
6 Cell membranes of human colorectal carcinomas and normal mucosa were prepared as described by Maeda et al with minor modifications .
7 Subcloning of specific restriction fragments were performed as described by Johansen ( 50 ) .
8 Otherwise the footprints were performed as described by Tullius and Dombroski ( 23 ) with the modifications developed by O'Halloran et al.
9 In vitro transcription assays were performed as described in Materials and Methods .
10 In vitro transcription reactions were performed as described in Materials and Methods .
11 Fragments RT , RN' and RK were sequenced as described under Materials and Methods .
12 Triglycerides were measured as described by Wahlefeld .
13 Purified MHC class II + epithelial cells were isolated as described in Fig.1 .
14 Signals obtained after hybridization of the RNA extracted from the two strains ( RNAs H/RNAs W ratios ) were compared as described in Material and Methods .
15 Trunk fragments with attached limbs ( +AER and -AER ) were cultured as described in the legend to Fig. 1 for 48h in serum-free medium supplemented as stated ( resupplemented after 24h ) .
16 Biotinylated anti-mouse immunoglobulins , streptavidin biotinylated peroxidase complex , DAB reaction , and the final counterstaining were conducted as described for the BrdU protocol .
17 Amylase content in the supernatant and dissolved pellet were determined as described by Bernfeld .
18 Deletions were cloned as described in Materials and Methods and transfected into F9 cells by electroporation .
19 To insert three or four foreign genes simultaneously into the AcNPV genome the recombinant vectors pAcAB3 and pAcAB4 were constructed as described in Methods .
20 The gels were loaded as described for the wild-type endonuclease ( figure 3 ) .
21 Various lines of transgenic mice bearing the -2300 bp human NF-l promoter deletion construct were established as described in Materials and Methods .
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