Example sentences of "was add [prep] each [noun sg] " in BNC.
Next page| No | Sentence |
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| 1 | DNA extraction — Sterile water ( 50 µl ) was added to each sample , which was then boiled for 30 minutes and a 3:2 ( vol:vol ) ratio of autoclaved 1 mm and 0.1 mm glass beads added to half fill each Eppendorf container ( Stratech Scientific , Luton ) . |
| 2 | Protein dissociation buffer ( 10% β-mercaptoethanol , 10% sodium dodecyl sulfate [ SDS ] , 25% glycerol , 10 mM Tris hydrochloride [ pH 6.8 ] , 0.02% bromophenol blue ) was added to each sample , and the mixture was incubated at 100°C for 5 min . |
| 3 | After incubation for 20 mins , 0.2 volumes of loading buffer ( 10mM Tris.HCl pH8.0 , 1mM EDTA , 10mM mercaptoethanol , 0.1% CHAPS , 50% glycerol and 0.25mg/ml bromophenol blue ) was added to each incubation mix . |
| 4 | The plates were then rapidly washed and 10 ml fresh αMEM with 1% FBS was added to each plate . |
| 5 | A separate ( 1,0 ) dummy was added for each quarter of the post-sample forecast and a test of their joint significance was undertaken . |