Example sentences of "then [vb past] [prep] the " in BNC.
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1 | We then checked over the cars and made an arrest . |
2 | Cells were treated with chemicals for 30min at 37 °C or as stated and then incubated for the indicated times before isolation of total RNA . |
3 | After blocking free protein binding sites with 0.5% Tween 20 ( Sigma ) for 2 hours , the filter was incubated with patient 's sera ( diluted 1:10 in TBS with 0.5% Tween 20 ) for 30 minutes , washed in 0.5% Tween in TBS , and then incubated with the rabbit anti-human immunoglobulin conjugated with alkaline phosphatase for a further 30 minutes . |
4 | Preparations were incubated with the primary antibody for 60 minutes , rinsed in Tris buffered saline and then incubated with the secondary antibody ( mouse anti-rabbit , diluted in Tris buffered saline/normal human serum ) for 30 minutes . |
5 | The sections were incubated in 5% normal rabbit serum to reduce background staining and then incubated with the monoclonal antibody to bromodeoxyuridine ( Dakopatts , Denmark ) diluted 1:100 with phosphate buffer for one hour . |
6 | Rather , they lived in practical uncertainty , often felt threatened , and then argued about the justice and permanence of the new order created for them . |
7 | Then argued about the wisdom of drinking before an overnight sail . |
8 | One presumes Waterloo then asked for the best of three for just qualifying for Bath is worth a minimum of £1,500 . |
9 | Yesterday 's court hearing came in the seventh week of the bitter dispute which began as a strike in protest over lay-offs then led to the sacking of 340 workers . |
10 | Yesterday 's hearing came in the seventh week of the dispute which began as a strike in protest over lay-offs then led to the sacking of 340 workers . |
11 | Mr Sloan then agreed to the postponement . |
12 | The Great Britain international hooker charged down full-back Garry Jack 's clumsy attempt to kick up field and then pounced over the line , Paul Eastwood adding the two points . |
13 | The sailors then regrouped and on Saturday marched on the Exchange , which then doubled as the Town Hall , demanding increased wages and paraded around the town . |
14 | I think he had a little bit of ill health and my immediate superior took over as Traffic Superintendent and he was there throughout the war and when I came out he was my boss and er you see and er and then in nineteen forty eight was made Transport Manager , because as you say we had to split from Electric Supply and he carried on until erm nineteen seventy two and erm , we had government reorganization and erm they did away with people like the Town Clerk and Transport Manager and erm erm was retired , early retirement , the same as the Town Clerk and erm they brought in a General Manager from away and brought in more staff with him and that was came in and er so I then applied for the position , which was going , there was , there was Traffic Superintendent was going er Chief Administration Officer , Chief Engineer and erm er Bodywork Maintenance Superintendent . |
15 | She then applied to the Secretary of State for payment under section 106 . |
16 | Once I had written these letters , I then applied to the Department that was officially responsible for serving the Diplomatic Corps . |
17 | It was over in a second , Barnett cannoned off the flattened front of the vehicle then tumbled down the road coming to rest in an inert heap . |
18 | I did a combined cycle and hiking day ; rode over the hill to Wainuiomata and down to the Rimutaka Forest Park , then tramped through the bush and up a long ridge to the top of Mount McKerrow . |
19 | The approaching star pulled out by gravity great elongated filaments of hot material into a curved configuration , which then condensed into the planets . |
20 | They were nervous all day and could n't concentrate on their schoolwork ; not even when Mr Litmus spilt a chemical on the floor and it started to fizzle , turning an incredible glowing blue , then splattered against the wall . |
21 | There he attracted his employer 's attention with a polite cough , then whispered in the latter 's ear : ‘ I 'm very sorry , sir , but there appears to be a tiger in the dining room . |
22 | Mr. Cowan then laid on the table the fifty volumes from Cassells Library which he presented to the Society . |
23 | type faces which can be stored on a disk and then downloaded to the printer when required for printing . |
24 | meetings with the Poultry Sub Committee which then reported to the Agriculture Sub Committee |
25 | Kenny then excelled in the higher sphere and helped the Palace to gain the 2nd Division championship with the club 's best-ever defensive record , and to reach the top of the Football League in September 1979 . |
26 | She then trained at the Medau School under the talented Irmela Doebner and some months after qualifying decided to come to England , and with the help of a postgraduate at Cambridge arranged to give a display at Newham College . |
27 | then dripped along the hall . |
28 | The long-range effects of mutations in the variable loop we then found for the synthesis of m 2 2G26 prompted us to investigate if also the formation of modifications in the anticodon loop were sensitive to point mutations situated far away in the tRNA . |
29 | A DEFLECTED shot from Scott Nisbet that spun off an opponent and then bounced over the head of a bemused Brugge goalkeeper last night kept alive Rangers ' hopes of a place in the European Cup final . |
30 | He jerked his head up , startled by her voice , then winced at the pain resulting from the sudden movement . |