Example sentences of "[noun pl] [be] [vb pp] for [adj] minutes " in BNC.
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1 | Muscle strips were precontracted by 10 µM acetylcholine and the mean amplitude of steady state contractions was measured for two minutes before and after the addition of leucocytes . |
2 | The mixtures were incubated for 30 minutes at 25°C and centrifuged for 10 minutes . |
3 | Pancreatic lysosomes from chow fed rats were incubated for 30 minutes at 37°C with ethanol , acetaldehyde or phosphatidylcholine vesicles containing cholesteryl oleate . |
4 | The supernatants were centrifuged for five minutes at 500 rpm and the epithelial cells contained in the pellets were treated with dispase ( Boehringer Mannheim Corp , Indianapolis , Indiana ) at a concentration of 3 mg/ml RPMI 1640 ( Whittaker Bioproducts , Walkersville , Maryland ) . |
5 | Before starting the study , pressures were recorded for 30 minutes to check the correct functioning of the recording system and to allow the subjects to accommodate to the catheters . |
6 | Suspensions were incubated for 5 minutes at 37°C . |
7 | Secretions were obtained for 60 minutes for estimation of basal acid output . |
8 | The pooled samples were homogenised for 20 minutes in five times the faecal weight of cold water using a Stomacher 3500 ( Seward , London , UK ) . |
9 | Samples were incubated for 10 minutes at 37°C before the reaction was stopped by removal of substrate and dilution by addition of 1.5 ml of 1:1 ( vol:vol ) H 2 O:Dowex-50W ( 200–400 , 8% cross linked , Na + -form ) . |
10 | One portion of the homogenates was boiled for 10 minutes , centrifuged at 5000 g for 10 minutes and the supernatant stored at -20°C for the later determination of histamine . |