Example sentences of "cell were [verb] by [adj] [noun sg] " in BNC.
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| 1 | Cryostat sections and cells were tested by indirect immunoperoxidase assays using rabbit anti-mouse antibody P260 Dakopatts ( Hamburg , Germany ) and the substrate 3-amino-9-ethylcarbazol Sigma ( Deisenhofen , Germany ) as an indicator system as described elsewhere . |
| 2 | Stromal cells were prepared by immunomagnetic selection from disaggregated 14-day fetal thymus lobes rendered free of lymphoid and major histocompatibility complex ( MHC ) class II + dendritic cells by culture in deoxyguanosine . |
| 3 | Activation of c-Myc was by addition of β -oestradiol ( 2μM ) to the culture medium , and cells were observed by time-lapse cinemicroscopy . |
| 4 | c-Myc was then activated by addition of β -oestradiol to a final concentration of 2μM and the cells were monitored by time-lapse cinemicroscopy at a rate of 12 frames per hour . |
| 5 | NIH3T3 cells were infected by overnight exposure to 0.45µM-filtered viral supernatants in the presence of 5µg/ml polybrene and colonies resistant to 1mg/ml G418 or 1.25 µg/ml puromycin were counted after 10-14 days . |
| 6 | Cells were lysed by repeated passage through a 19-gauge needle . |
| 7 | To assess whether these multiple recombinant baculoviruses synthesized BTV proteins in S.frugiperda cells , RNAs and mRNAs made in infected cells were identified by Northern Blot hybridization analysis ( data not shown ) , and the proteins made in infected cells were analysed by SDS-PAGE as described in Methods . |
| 8 | BL21(DE3) cells were transformed by each plasmid and the cells were grown at 37°C in an LB medium containing 100µg/ml ampicillin until A 600 reached 0.6 , at which time IPTG was added to a final concentration of 1.0mM and the cells were allowed to grow for an additional 3 hours . |
| 9 | This suggests that either the K + channel bearing cells were overgrown by other cell types when true confluency was achieved , or confluency was associated with full polarisation of the cells and mvoement of K + channels to the basolateral membrane , which was inaccessible to the patch pipette . |